Ultra-thin Serial Sectioning with Rocking Microtomes
نویسندگان
چکیده
منابع مشابه
Three-dimensional localisation of DNA in the nucleolus of Spirogyra by correlated optical tomography and serial ultra-thin sectioning.
Spirogyra nucleoli were shown by three-dimensional optical microscopy of DAPI fluorescence to contain DNA with a pattern and distribution matching those of the fibrillar centres. This was confirmed using different species with nucleoli showing different sizes of fibrillar centre. Much lower levels of fluorescence were seen corresponding to the dense fibrillar component. Nearly all the DAPI fluo...
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Kristen M. Harris,1,2,3 Elizabeth Perry,2 Jennifer Bourne,1,2 Marcia Feinberg,3 Linnaea Ostroff,3 and Jamie Hurlburt2,3 1Section of Neurobiology and Center for Learning and Memory, University of Texas at Austin, Austin, Texas 78712, 2Department of Neurology, Synapses, and Cognitive Neuroscience Center, Medical College of Georgia, Augusta, Georgia 30912, 3Program in Neuroscience and Biology Depa...
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The study of cytological fine structure with the electron microscope, has as an important prerequisite, the ability to cut consistently good quality thin sections. Satisfactory techniques for doing this are well known in many laboratories and an excellent review of the subject has recently been published by Farquhar (1). However, some of the details of the practice of microtomy are not in the l...
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ژورنال
عنوان ژورنال: Nature
سال: 1957
ISSN: 0028-0836,1476-4687
DOI: 10.1038/1801302a0